The alleged incident occurred Friday at a central-area residence in Sault Ste. Marie. All three players are accused of having sexual intercourse with the female.
The female reported the alleged incident to police, charges were laid Saturday and details were released Sunday.
Petaccio, an American-born player from Sicklerville, New Jersey, appeared in Sault Ste. Marie bail court on Monday morning.
In a press release issued on Saturday night, the Greyhound organization noted how it has seen the three players "make excellent strides both on and off the ice."
"That's what makes this surprising," Dubas added. "This is going to go through the legal process. This is an accusation and the players will have the opportunity to defend themselves. We'll continue to perform our duty and support these players."
As part of their press release, the Greyhounds said they would continue to support the police investigation "in any way possible."
The Greyhounds organization informed Sault Ste. Marie media outlets that both Cousins and Petaccio were directing all inquiries to their respective agents. Fritsch's parents issued a statement saying their son is not represented by an agent and the family is seeking the advice of legal counsel.
Petaccio's agent, David Maciuk, a former Greyhound player himself from the early 1970s, said: "We're just gathering information and will not make comment until we know more."
Maciuk, who was born and raised in Sault Ste. Marie, has lived in the Chicago area for more than 35 years.
Ottawa-based player agent Larry Kelly, who represents Cousins, said on Monday that "as of now, I can't make any comment."
Cousins, a third-round draft choice of Philadelphia Flyers in 2011, is under contract to the National Hockey League team and is considered one of its top junior-aged prospects.
"We are aware of the unfortunate incident involving Nick Cousins," Philadelphia Flyers general manager Paul Holmgren said via press release. "Due to a lack of information available to us, we will not comment at this time."
Fritsch is also a 2011 NHL draft pick, having been selected by Phoenix Coyotes in the sixth round. He has not signed an NHL contract.
As the result of being charged by police, the Greyhounds could find themselves minus the three players during games against the OHL's three American-based teams: Erie Otters, Plymouth Whalers and Saginaw Spirit.
"A conviction would most-definitely keep the individuals in question out of the United States," a Sault Ste. Marie, Michigan-based immigration official, speaking on the condition of anonymity, told Juniorhockey.com. "In all cases, with people who've been charged but who are awaiting court dates, we look at the cases on an individual basis."
"Bail conditions would have to be looked at before they could enter the United States," the immigration official added. "The players in question could be allowed to cross into the United States from Canada but we'd have to ascertain whether they'd be a flight risk."